Stewart Research Group
    Department of Chemistry, University of Florida
    Complete list of publications since 1994
   
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Biocatalytic reductions
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Antibodies
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Review articles & book chapters
  1. Chemistry for a Sustainable Future.  Grassian, V.H.; Meyer, G.; H. Abruña, H., G.W. Coates, L.E. Achenie, T. Allison, B. Brunschwig, J. Ferry, M. Garcia-Garibay, J. Gardea-Torresdaey, C.P. Grey, J. Hutchison, C.-J. Li, C. Liotta, A. Ragauskas, S. Minteer, K. Mueller, J. Roberts, O. Sadik, R. Schmehl, W. Schneider, A. Selloni, P. Stair, J. Stewart, D. Thorn, J. Tyson, B. Voelker, J.M. White and F. Wood-Black, Environ. Sci. Technol. 2007, 4840-4846.
  2. Biofunctionalization and Capping of Template Synthesized Nanotubes.  H. Hillebrenner, F. Buyukserin, J.D. Stewart and C.R. Martin, J. Nanosci. Nanotechnol. 2007, 7, 2211-2221.
  3. Future Directions in Alcohol Dehydrogenase-Catalyzed Reactions.  J.D. Stewart, in Future Directions in Biocatalysis, Matsuda, T., Ed., 2007, San Diego:  Elsevier, in press.
  4. A Genomic Approach to Investigating Bakers' Yeast Reductions.  J.D. Stewart, in Biocatalysis in the Pharmaceutical and Biotechnological Industries, Patel, R.N. Ed., 2007, New York:  Dekker, pp. 333-350.
  5. Genomes as Resources for Biocatalysis.  J.D. Stewart, in Advances in Microbiology, Sariaslani, S., Ed., 2006, San Diego:  Elsevier, 59, 31-52.
  6. Green Chemical Manufacturing with Biocatalysis.  J.D. Stewart, in Environmental Catalysis, V.H. Grassian, Ed., Boca Raton, FL:  CRC Press, 2004, pp. 649-665.
  7. Enantio Enriched Substituted Polycaprolactones by Enzyme Catalysis.  K.S. Bisht, L. Kondaveti, and J.D. Stewart, ACS Symposium Series 900, H.N. Cheng and R.A. Gross, Eds., Washington, DC:  American Chemical Society, 2004, pp. 366-392.
  8. Cloning, Structure and Activity of Ketone Reductases from Baker’s Yeast.  J.D. Stewart, S. Rodriguez and M.M. Kayser, in Enzyme Technology for Pharmaceutical and Biotechnological Applications, H.A. Kirst, W.-K. Yeh and M.J. Zmijewski, eds, New York:  Marcel Dekker, 2001, pp. 175-207.
  9. Filamentous Fungi:  Potentially Useful Catalysts for the Biohydroxylations of Non-Activated Carbon Centers.  L.R. Lehman and J.D. Stewart, Curr. Org. Chem., 2001, 5, 439-470.
  10. Dehydrogenases and Transaminases in Asymmetric Synthesis.  J.D. Stewart, Curr. Opin. Chem. Biol., 2001, 5, 120-129.
  11. Organic Transformations Catalyzed by Engineered Yeast Cells and Related Systems.  J.D. Stewart, Curr. Opinion Biotechnol., 2000, 11, 363-368.
  12. Cytochrome P450’s:  Potential Catalysts for Asymmetric Olefin Epoxidations.  C.A. Martinez and J.D. Stewart, Curr. Org. Chem., 2000, 4, 263-282.
  13. 'Designer Yeast':  An Enantioselective Oxidizing Reagent for Organic Synthesis.  M.M. Kayser, G. Chen and J.D. Stewart, Synlett, 1999, 153-158.
  14. Baker’s Yeast Reductions in Asymmetric Synthesis. J.D. Stewart, Curr. Opinion in Drug Discovery and Development, 1998, 1, 278-289.
  15. Cyclohexanone Monooxygenase:  A Useful Reagent for Asymmetric Baeyer-Villiger Reactions.J.D. Stewart, Curr. Org. Chem. 1998, 2, 211-232.
  16. A Chemist's Perspective on the Use of Genetically Engineered Microbes as Reagents for Organic Synthesis. J.D. Stewart, Biotechnol. Genetic Eng. Rev., 1997, 14, 67-143.
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Biocatalytic reductions
  1. Site Saturation Mutagenesis of Tryptophan 116 of Saccharomyces pastorianus Old Yellow Enzyme Uncovers Stereocomplementary Variants.  S.K. Padhi, D.J. Bougioukou and J.D. Stewart, J. Am. Chem. Soc. 2009, 131, 3271-3280.

  2. Opposite Stereochemical Courses for Enzyme-Mediated Alkene Reductions of an Enantiomeric Substrate Pair.  D.J. Bougioukou and J.D. Stewart, J. Am. Chem. Soc., 2008, 130, 7655-7658.
  3. Reductions of Cyclic beta-Keto Esters by Individual Saccharomyces cerevisiae Dehydrogenases and a Chemo-Enzymatic Route to (1R,2S)-2-Methyl-1-cyclohexanol.  S.K. Padhi, I.A. Kaluzna, D. Buisson, R. Azerad and J.D. Stewart, Tetrahedron:  Asymmetry 2007, 18, 2133-2138.
  4. Asymmetric Bioreductions of Beta-Nitroacrylates as a Route to Beta2-Amino Acids.  M.A. Swiderska and J.D. Stewart, Org. Lett. 2006, in press.
  5. Stereoselective Enone Reductions by Saccharomyces carlsbergensis Old Yellow Enzyme.  M.A. Swiderska and J.D. Stewart, J. Mol. Catal. B:  Enzymatic 2006, in press.
  6. Application of Newly-Available Bio-Reducing Agents to the Synthesis of Chiral Hydroxy-beta-Lactams; Model for Aldose Reductase Stereochemistry.  M.M. Kayser, M. Drolet and J.D. Stewart, Tetrahedron:  Asymmetry 2005, 16, 4004-4009.
  7. Enantiodivergent, Biocatalytic Routes to Both Taxol Side Chain Antipodes.  B.D. Feske, I.A. Kaluzna and J.D. Stewart, J. Org. Chem. 2005, 70, 9654-9657.
  8. Chemoenzymatic Formal Total Synthesis of (-)-Bestatin.  B.D. Feske and J.D. Stewart, Tetrahedron:  Asymmetry 2005, 16, 3124-3127.
  9. Assessing Substrate Acceptance and Enantioselectivity of Yeast Reductases in Reactions with Substituted alpha-Keto-beta-Lactams.  Y.Yang, M.M. Kayser, F.D. Rochon, S. Rodriguez and J.D. Stewart, J. Mol. Catal. B:  Enzymatic 2005, 32, 167-174.
  10. Stereoselective, Biocatalytic Reductions of alpha-Chloro-beta-Keto Esters.  I.A. Kaluzna, W. Wittayanan, B.D. Feske, I. Ghiviriga and J.D. Stewart, J. Org. Chem. 2005, 70, 342-345.
  11. A Systematic Investigation of Saccharomyces cerevisiae Enzymes Catalyzing Carbonyl Reductions.  I.A. Kaluzna, T. Matsuda, A.K. Sewall and J.D. Stewart, J. Am. Chem. Soc. 2004, 126, 12827-12832.
  12. Regio- and Enantioselective Reduction of t-Butyl 6-Chloro-3,5-dioxohexanoate With Baker’s Yeast.  M. Wolberg, I.A. Kaluzna, M. Mueller and J.D. Stewart, Tetrahedron:  Asymmetry 2004, 15, 2825-2828.
  13. Enantioselective Reductions of Ethyl 2-Oxo-4-phenylbutyrate by Saccharomyces cerevisiae Dehydrogenases.  I. Kaluzna, A.A. Andrew, M. Bonilla, M.R. Martzen and J.D. Stewart, J. Mol. Catal. B:  Enzymatic, 2002, 17, 101-105.
  14. Purification and Identification of an Escherichia coli beta-Keto Ester Reductase as 2,5-Diketo-D-gluconate Reductase YqhE.  M. Habrych, S. Rodriguez and J.D. Stewart, Biotechnol. Progress 2002, 18, 257-261.
  15. Highly Stereoselective Reagents for beta-Keto Ester Reductions by Genetic Engineering of Baker’s Yeast.  S. Rodriguez, M.M. Kayser and J.D. Stewart, J. Am. Chem. Soc., 2001, 123, 1547-1555.
  16. Asymmetric Synthesis of beta-Hydroxy Esters and alpha-Alkyl-beta-hydroxy Esters by Recombinant Escherichia coli Expressing Enzymes from Baker’s Yeast.  S. Rodriguez, K.T. Schroeder, M.M. Kayser and J.D. Stewart, J. Org. Chem., 2000, 65, 2586-2587.
  17. Improving the Stereoselectivity of Baker’s Yeast Reductions by Genetic Engineering.  S. Rodriguez, M.M. Kayser and J.D. Stewart, Org. Lett., 1999, 1, 1153-1155.
  18. Baker’s Yeast-Mediated Reductions of alpha-Keto Esters and an alpha-Keto-beta-Lactam.  Two Routes to the Paclitaxel Side Chain. M.M. Kayser, M.D. Mihovilovic, J. Kearns, A. Feicht and J.D. Stewart, J. Org Chem., 1999, 64, 6603-6608.
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Biocatalytic oxidations
  1. Understanding and Improving NADPH-Dependent Reactions by Non-Growing Escherichia coli Cells.  A.Z. Walton and J.D. Stewart, Biotechnol. Progress 2004, 20, 403-411.
  2. Assessing the Substrate- and Enantioselectivities of Eight Novel Baeyer-Villiger Monooxygenases Toward Alkyl-Substituted Cyclohexanones.  B.G. Kyte, P. Rouviere, Q. Cheng and J.D. Stewart, J. Org. Chem., 2004, 69, 12-17.
  3. Whole-Cell Mediated Baeyer-Villiger Oxidation of Functionalized Bicyclo[3.3.0]ketones by Recombinant E. coli.  M.D. Mihovilovic, B. Mueller, M.M. Kayser, J.D. Stewart and P. Stanetty, Synlett 2002, 703-706.
  4. An Efficient Enzymatic Baeyer-Villiger Oxidation by Engineered Escherichia coli Cells Under Non-Growing Conditions.  A.Z. Walton and J.D. Stewart, Biotechnol. Progress 2002, 18, 262-268.
  5. Biocatalytic Lactone Production in Genetically Engineered E. coli and Identification by Gas Chromatography and Mass Spectroscopy.  C. Slawson, J.D. Stewart and R. Potter, J. Chem. Educ.2001, 78, 1533-1534.
  6. Asymmetric Baeyer-Villiger Oxidations of 4-Mono- and 4,4-Disubstituted Cyclohexanones by Whole Cells of Engineered Escherichia coli.  M.D. Mihovilovic, G. Chen, S. Wang, B. Kyte, F. Rochon, M.M. Kayser and J.D. Stewart, J. Org. Chem., 2001, 66, 733-738.
  7. Baeyer-Villiger Oxidations of Representative Heterocyclic Ketones by Whole Cells of Engineered Escherichia coli Expressing Cyclohexanone Monooxygenase.  M.D. Mihovilovic, B. Mueller, M.M. Kayser, J.D. Stewart, J. Fröhlich, P. Stanetty, and H. Spreitzer, J. Mol. Catal. B:  Enzymatic, 2001, 11, 349-353.
  8. Asymmetric Oxidations at Sulfur Catalyzed by Engineered Strains That Overexpress Cyclohexanone Monooxygenase.  G. Chen, M.M. Kayser, M.D. Mihovilovic, M.E. Mrstik, C.A. Martinez and J.D. Stewart, New J. Chem., 1999, 827-832.
  9. Enantio- and Regioselective Baeyer-Villiger Oxidations of 2- and 3-Substituted Cyclopentanones Using “Designer” Oxidizing Yeast.  M.M. Kayser, G. Chen and J.D. Stewart, J. Org. Chem., 1998, 63, 7103-7106.
  10. Recombinant Baker’s Yeast as a Whole-Cell Catalyst for Asymmetric Baeyer-Villiger Oxidations.  J.D. Stewart, K.W. Reed, C.A. Martinez, J. Zhu, G. Chen and M.M. Kayser, J. Am. Chem. Soc., 1998, 120, 3541-3548.
  11. A “Designer Yeast” That Catalyzes the Kinetic Resolutions of 2-Alkyl-Substituted Cyclohexanones by Enantioselective Baeyer-Villiger Oxidations.  J.D. Stewart, K.W. Reed, J. Zhu, G. Chen and M.M. Kayser, J. Org. Chem., 1996, 61, 7652-7653.
  12. 'Designer Yeast':  A New Reagent for Enantioselective Baeyer-Villiger Oxidations.  J.D. Stewart, K.W. Reed and M.M. Kayser, J. Chem. Soc. Perkin Trans. 1, 1996, 755-757.
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Antibodies
  1. Analysis of Molecular Recognition of Antibodies Specific for UV-Damaged DNA.  H. Morioka, H. Kobayashi, M. Kurihara, J. Kato, Y. Komatsu, K. Sato, K. Nobuoka, K. Kato, T. Torizawa, I. Shimada, Y. Satow, J.D. Stewart, T. Matsunaga, O. Nikaido and E. Ohtsuka, Photomed. Photobiol. 1999, 21, 7-8.
  2. Tryptophan H33 Plays an Important Role in Pyrimidine (6-4) Pyrimidone Photoproduct Binding by a High-Affinity Antibody.  H. Kobayashi, J. Kato, H. Morioka, J.D. Stewart and E. Ohtsuka, Protein Eng., 1999, 12, 879-884.
  3. Crystallographic Structures of the Amide-Hydrolyzing Catalytic Antibody 43C9.  M.M. Thayer, E.H. Olender, A.S. Arvei, I.S. Canestrelli, J.D. Stewart, S.J. Benkovic, E.D. Getzoff and V.A. Roberts, J. Mol. Biol., 1999, 291, 329-345.
  4. Effects of a High-Affinity Antibody Fragment on DNA Polymerase Reactions Near a (6-4) Photoproduct Site.  H. Kobayashi, K. Sato, Y. Komatsu, H. Morioka, J.D. Stewart, T. Tsurimoto and E. Ohtsuka, Photochem. Photobiol., 1999, 69, 226-230.
  5. Probing the Interaction Between a High-Affinity Single-Chain Fv and a Pyrimidine (6-4) Pyrimidone Photodimer by Site-Directed Mutagenesis.  H. Kobayashi, H. Morioka, K. Tobisawa, T. Torizawa, K. Koto, I. Shimada, O. Nikaido, J.D. Stewart and E. Ohtsuka, Biochemistry, 1999, 38, 532-539.
  6. The Role of Surface Lysines in Pyrimidine (6-4) Pyrimidone Photoproduct Binding by a High-Affinity Antibody.  H. Kobayashi, H. Morioka, O. Nikaido, J.D. Stewart and E. Ohtsuka, Protein Eng., 1998, 11, 1089-1092.
  7. Antibodies Specific for (6-4) DNA Photoproducts:  Cloning, Antibody Modeling and Construction of a Single-Chain Fv Derivative.  H. Morioka, H. Miura, H. Kobayashi, T. Koizumi, K. Fujii, K. Asano, T. Matsunaga, O. Nikaido, J.D. Stewart and E. Ohtsuka, Biochim. Biophys. Acta, 1998, 1385, 17-32.
  8. Transition State Stabilization as a Measure of the Efficiency of Antibody Catalysis.  J.D. StewartS.J. Benkovic, Nature, 1995, 375, 388-391. and
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Other projects
  1. Characterization of an Autonomously Activated Plant Adenosine Diphosphate Glucose Pyrophosphorylase.  S.K. Boehlein, J.R. Shaw, J.D. Stewart and L.C. Hannah, Plant Physiol. 2009, 149, 318-326.
  2. Heat Stability and Allosteric Properties of the Maize Endosperm ADP-Glucose Pyrophosphorylase are Intimately Intertwined.  S.K. Boehlein, J.R. Shaw, J.D. Stewart| and L.C. Hannah, Plant Physiol. 2008, 146, 289-299.
  3. Template Synthesized Nanotubes for Biomedical Delivery Applications.  H. Hillebrenner, F. Buyukserin, J.D. Stewart and C.R. Martin, Nanomedicine 2006, 1, 39-50.
  4. Corking Nano Test Tubes by Chemical Self Assembly.  H. Hillebrenner, M. Kang, F. Buyukserin, M.O. Mota, J.D. Stewart and C.R. Martin, J. Am. Chem. Soc. 2006, 128, 4236-4237.
  5. Purification and Characterization of Adenosine Diphosphate Glucose Pyrophosphorylase from Maize/Potato Mosaics.  S.K. Boehlein, A.K. Sewell, J. Cross, J.D. Stewart and L.C. Hannah, Plant Physiol. 2005, 138, 1552-1562.
  6. gamma-Glutamyl Thioester Intermediate in Glutaminase Reaction Catalyzed by Escherichia coli Asparagine Synthetase B.  H.G. Schnizer, S.K. Boehlein, J.D. Stewart, N.G.J. Richards and S.M. Schuster.  Methods Enzymol. 2002, 354, 260-271.
  7. Characterization of Inhibitors Acting at the Synthetase Site of Escherichia coli Asparagine Synthetase B.  S.K. Boehlein, T. Nakatsu, J. Hiratake, R. Thirumoorthy, J.D. Stewart, N.G.J. Richards and S.M. Schuster, Biochemistry, 2001, 40, 11168-11175.
  8. Formation and Isolation of a Covalent Intermediate During the Glutaminase Reaction of a Class II Amidotransferase.  H.G. Schnizer, S.K. Boehlein, J.D. Stewart, N.G.J. Richards and S.M. Schuster, Biochemistry, 1999, 38, 3677-3682.
  9. The Kinetic Mechanism of E. coli Asparagine Synthetase B.  S.K. Boehlein, J.D. Stewart, E.S. Walworth, R. Thiramoorthy, N.G.J. Richards and S.M. Schuster, Biochemistry 1998, 37, 13230-13238.

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Last updated 5/5/09 by J.D.S.